Surowiec, Izabella, Trojanowicz, Marek and Quye, Anita (2006) Liquid chromatography determination of natural dyes in extracts from historical Scottish textiles excavated from peat bogs. Journal of Chromatography A, 1112 (1-2). pp. 209-217. ISSN 0021-9673

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Textiles excavated from Scottish sites belonging now to the collections of the National Museums of Scotland, including seventeenth century textiles from peat bogs in the Scottish Highlands and Islands, were selected for analysis by high performance liquid chromatography with photodiode array detection (PDA HPLC) to detect whether any dyes remained and, if so, to identify their biological sources. Dye components were identified in 36 of the 81 samples analysed. Although it was not possible to identify the exact sources of the dyestuffs because of the wide-spread occurrence of these natural dyes components, the study has shown that textiles previously not thought to have been coloured had detectable traces of dye. Before the historical textiles were analyzed, an improved extraction procedure that combined the routine acid hydrolysis method with one using dimethylformamide (DMF) was applied. The DMF method enabled increased recovery of major flavonoid and anthraquinoid compounds, and very high efficiency of recovery of indigotin even in textiles with no colour visible, thereby complementing the acid hydrolysis method already in use. Extracts from historical thread samples were analysed by PDA HPLC using a reversed-phase gradient system comprising of a C18 column (150 mm × 4.6 mm i.d., 25 ± 1 °C) with water, methanol and o-phosphoric acid at an eluent flow rate of 1.2 ml/min. A preliminary investigation to improve the detection limits further for a selection of natural dyes was made by comparing results from the 4.6 mm internal diameter (i.d.) column with a narrow bore C18 column (2.1 mm i.d.). An increase in the detector response was observed for narrow-bore column proving its possibility of enhancement of sensitivity

Item Type: Article
Additional Information: Natural dyes; HPLC; Photodiode array detection; Extraction; Scottish textiles
Subjects: A General Works > AM Museums (General). Collectors and collecting (General)
C Auxiliary Sciences of History > CC Archaeology
D History General and Old World > DA Great Britain
N Fine Arts > NK Decorative arts Applied arts Decoration and ornament
Q Science > Q Science (General)
Q Science > QD Chemistry
Theme: Collections & collecting
Department: Conservation and Analytical Research
Related URLs:
Depositing User: Ross Anderson
Date Deposited: 21 Mar 2019 17:23
Last Modified: 21 Mar 2019 17:23

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